Journal: Nature

Article Title: Na v 1.7 as a chondrocyte regulator and therapeutic target for osteoarthritis.

doi: 10.1038/s41586-023-06888-7

Figure Lengend Snippet: Fig. 3 | Blockade of Nav1.7 regulates chondrocyte biology through enhancing HSP70 and midkine secretion. a, Peptide-spectrum match (PSM)-based abundance of proteins identified from the 30–100 kDa fraction of conditioned medium that are unique to (red dots) or increased with (blue dots) PF-04856264 and ProTx II treatment. b, PSM-based abundance of proteins (red dot) identified from the 10–30 kDa fraction of conditioned medium that are unique to PF-04856264 and ProTx II treatment. c, COL2 and ACAN mRNA levels in human C28I2 chondrocytes stimulated with conditioned medium collected from cells treated with ProTx II (Pro-CM) or PF-04856264 (PF-CM) in the absence or presence of IgG or anti-HSP70 antibodies (n = 4 biological replicates). d, MMP13 and ADAMTS5 mRNA levels in C28I2 chondrocytes stimulated with IL-1β and

Article Snippet: Human OA chondrocytes or C28I2 cells were seeded on 8-well chamber (Thermo Fisher, 154461) and loaded with 5 μM CoroNa Green (Invitrogen, C36676) or Fluo-8 (Abcam, ab112129) in Hanks’ Balanced Salt Solution for 45 min at 37 °C in the presence or absence of 25 nM ProTx II or 1 μM PF-04856264 with or without 0.5 μM KB-R7943 (Tocris, 1244).

Techniques: